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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha : |
31/07/2018 |
Actualizado : |
11/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
DOSTER, E.; ROVIRA, P.J.; NOYES, N.R.; BURGESS, B. A.; YANG, X.; WEINROTH, M.D.; LAKIN, S.M.; DEAN, C.J.; LINKE, L.; MAGNUSON, R.; JONES, K.I.; BOUCHER, C.; RUIZ, J.; BELK, K.E.; MORLEY, P.S. |
Afiliación : |
ENRIQUE DOSTER, Microbial Ecology Group, Colorado State University, USA. Department of Microbiology, Inmunology and Pathology, Colorado State University. USA.; PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Microbial Ecology Group, Colorado State University. Department of Microbiology, Imnunology and Pathology, Colorado State University, USA.; NOELLE R. NOYES., Microbial Ecology Group, Colorado State University. Department of Veterinary Population Medicine, University of Minnesota.; BRANDY A. BURGESS, Department of Population Health, University of Georgia, USA.; XIANG YANG, Microbial Ecology Group, Colorado State University. Department of Animal Sciences, Colorado State University, USA.; MARGARET D. WEINROTH, Microbial Ecology Group, Colorado State University. Department of Animal Sciences, Colorado State University, USA.; STEVEN M. LAKIN, Microbial Ecology Group, Colorado State University. Department of Microbiology, Immunology and Pathology, Colorado State University, USA.; CHRISTOPHER J. DEAN, Microbial Ecology Group, Colorado State University. Department of Microbiology, Immunology and Pathology, Colorado State University, USA.; LYNDSEY LINKE, Department of Clinical Sciences, Colorado State University, USA.; ROBERTA MAGNUSON, Department of Clinical Sciences, Colorado State University, USA.; KENNETH I. JONES, Department of Biochemistry and Molecular Genetics, University of Colorado, USA.; CHRISTINA BOUCHER, Department of Computer and Information Science and Engineering, University of Florida, USA.; JAMIE RUIZ, Department of Computer and Information Science and Engineering, University of Florida, USA.; KEITH E. BELK, Microbial Ecology Group, Colorado State University. Department of Microbiology, Imnunology and Pathology, Colorado State University, USA.; PAUL S. MORLEY, Microbial Ecology Group, Colorado State University, USA. Department of Microbiology, Inmunology and Pathology, Colorado State University. USA. |
Título : |
Investigating effects of tulathromycin metaphylaxis on the fecal resistome and microbiome of commercial feedlot cattle early in the feeding period. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Frontier in Microbiology, 2018, 9:1715. |
Páginas : |
14 p. |
DOI : |
10.3389/fmicb.2018.01715 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 14 April 2018; Accepted: 09 July 2018; Published: 30 July 2018.
Open Access journal.
https://doi.org/10.3389/fmicb.2018.01715 |
Contenido : |
The objective was to examine effects of treating commercial beef feedlot cattle with therapeutic doses of tulathromycin, a macrolide antimicrobial drug, on changes in the fecal resistome and microbiome using shotgun metagenomic sequencing. Two pens of cattle were used, with all cattle in one pen receiving metaphylaxis treatment (800 mg subcutaneous tulathromycin) at arrival to the feedlot, and all cattle in the other pen remaining unexposed to parenteral antibiotics throughout the study period. Fecal samples were collected from 15 selected cattle in each group just prior to treatment (Day 1), and again 11 days later (Day 11). Shotgun sequencing was performed on isolated metagenomic DNA, and reads were aligned to a resistance and a taxonomic database to identify alignments to antimicrobial resistance (AMR) gene accessions and microbiome content. Overall, we identified AMR genes accessions encompassing 9 classes of AMR drugs and encoding 24 unique AMR mechanisms. Statistical analysis was used to identify differences in the resistome and microbiome between the untreated and treated groups at both timepoints, as well as over time. Based on composition and ordination analyses, the resistome and microbiome were not significantly different between the two groups on Day 1 or on Day 11. However, both the resistome and microbiome changed significantly between these two sampling dates. These results indicate that the transition into the feedlot?and associated changes in diet, geography, conspecific exposure, and environment?may exert a greater influence over the fecal resistome and microbiome of feedlot cattle than common metaphylactic antimicrobial drug treatment. MenosThe objective was to examine effects of treating commercial beef feedlot cattle with therapeutic doses of tulathromycin, a macrolide antimicrobial drug, on changes in the fecal resistome and microbiome using shotgun metagenomic sequencing. Two pens of cattle were used, with all cattle in one pen receiving metaphylaxis treatment (800 mg subcutaneous tulathromycin) at arrival to the feedlot, and all cattle in the other pen remaining unexposed to parenteral antibiotics throughout the study period. Fecal samples were collected from 15 selected cattle in each group just prior to treatment (Day 1), and again 11 days later (Day 11). Shotgun sequencing was performed on isolated metagenomic DNA, and reads were aligned to a resistance and a taxonomic database to identify alignments to antimicrobial resistance (AMR) gene accessions and microbiome content. Overall, we identified AMR genes accessions encompassing 9 classes of AMR drugs and encoding 24 unique AMR mechanisms. Statistical analysis was used to identify differences in the resistome and microbiome between the untreated and treated groups at both timepoints, as well as over time. Based on composition and ordination analyses, the resistome and microbiome were not significantly different between the two groups on Day 1 or on Day 11. However, both the resistome and microbiome changed significantly between these two sampling dates. These results indicate that the transition into the feedlot?and associated changes in diet, geography... Presentar Todo |
Palabras claves : |
METAGENOMICS; METAPHYLAXIS; MICROBIOME; RESISTOME; TULATHROMYCIN. |
Thesagro : |
BOVINOS; FEEDLOT. |
Asunto categoría : |
L73 Enfermedades de los animales |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10933/1/fmicb-09-01715.pdf
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Marc : |
LEADER 02931naa a2200409 a 4500 001 1058855 005 2019-10-11 008 2018 bl uuuu u00u1 u #d 024 7 $a10.3389/fmicb.2018.01715$2DOI 100 1 $aDOSTER, E. 245 $aInvestigating effects of tulathromycin metaphylaxis on the fecal resistome and microbiome of commercial feedlot cattle early in the feeding period.$h[electronic resource] 260 $c2018 300 $a14 p. 500 $aArticle history: Received: 14 April 2018; Accepted: 09 July 2018; Published: 30 July 2018. Open Access journal. https://doi.org/10.3389/fmicb.2018.01715 520 $aThe objective was to examine effects of treating commercial beef feedlot cattle with therapeutic doses of tulathromycin, a macrolide antimicrobial drug, on changes in the fecal resistome and microbiome using shotgun metagenomic sequencing. Two pens of cattle were used, with all cattle in one pen receiving metaphylaxis treatment (800 mg subcutaneous tulathromycin) at arrival to the feedlot, and all cattle in the other pen remaining unexposed to parenteral antibiotics throughout the study period. Fecal samples were collected from 15 selected cattle in each group just prior to treatment (Day 1), and again 11 days later (Day 11). Shotgun sequencing was performed on isolated metagenomic DNA, and reads were aligned to a resistance and a taxonomic database to identify alignments to antimicrobial resistance (AMR) gene accessions and microbiome content. Overall, we identified AMR genes accessions encompassing 9 classes of AMR drugs and encoding 24 unique AMR mechanisms. Statistical analysis was used to identify differences in the resistome and microbiome between the untreated and treated groups at both timepoints, as well as over time. Based on composition and ordination analyses, the resistome and microbiome were not significantly different between the two groups on Day 1 or on Day 11. However, both the resistome and microbiome changed significantly between these two sampling dates. These results indicate that the transition into the feedlot?and associated changes in diet, geography, conspecific exposure, and environment?may exert a greater influence over the fecal resistome and microbiome of feedlot cattle than common metaphylactic antimicrobial drug treatment. 650 $aBOVINOS 650 $aFEEDLOT 653 $aMETAGENOMICS 653 $aMETAPHYLAXIS 653 $aMICROBIOME 653 $aRESISTOME 653 $aTULATHROMYCIN 700 1 $aROVIRA, P.J. 700 1 $aNOYES, N.R. 700 1 $aBURGESS, B. A. 700 1 $aYANG, X. 700 1 $aWEINROTH, M.D. 700 1 $aLAKIN, S.M. 700 1 $aDEAN, C.J. 700 1 $aLINKE, L. 700 1 $aMAGNUSON, R. 700 1 $aJONES, K.I. 700 1 $aBOUCHER, C. 700 1 $aRUIZ, J. 700 1 $aBELK, K.E. 700 1 $aMORLEY, P.S. 773 $tFrontier in Microbiology, 2018, 9:1715.
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INIA Treinta y Tres (TT) |
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
22/12/2020 |
Actualizado : |
12/03/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
-- - -- |
Autor : |
FORT, S.; FERREIRA, V.; MURCHIO, S.; SCHVARTZMAN, C.; GALVÁN, G.A.; VILARÓ, F.; SIRI, M.I.; DALLA RIZZA, M. |
Afiliación : |
S. FORT, Universidad de la República, Facultad de Química. Laboratorio de Microbiología Molecular, Uruguay.; V. FERREIRA, Universidad de la República, Facultad de Química. Laboratorio de Microbiología Molecular, Uruguay.; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; G.A. GALVÁN, Universidad de la República. Facultad de Agronomía. Departamento de Producción Vegetal.; F. VILARÓ, Universidad de la República. Facultad de Agronomía. Departamento de Producción Vegetal.; M.I. SIRI, Universidad de la República. Facultad de Química. Laboratorio de Microbiología Molecular.; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Potato plants transformed with the Arabidopsis EF-Tu receptor (EFR) show restricted pathogen colonization and enhanced bacterial with resistance under conditions resembling natural field infections. [Plantas de papa transformadas con el receptor de EF-Tu (EFR) de Arabidopsis presentan una colonización restringida del patógeno y mayor resistencia a la marchitez Bacteriana bajo condiciones semejantes a la infección natural de campo]. |
Complemento del título : |
Special Issue X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria; XII Simposio REDBIO Argentina. Roca, William, Ed. 12 al 15 de noviembre de 2019, Montevideo, Uruguay. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Agrociencia Uruguay 2020, v. 24, no. NE2, Article 413. DOI: 10.31285/AGRO.24.413 |
Páginas : |
16 p. |
Serie : |
2301-1548 |
DOI : |
10.31285/AGRO.24.413 |
Idioma : |
Inglés |
Notas : |
Article history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020.
Comité científico editor:
Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad Nacional de la Plata, La Plata, Buenos Aires, Argentina); Dra. Alicia Castillo (Investigadora Principal, Unidad de Biotecnología de INIA, Uruguay) ; Dr. Juan Izquierdo (Profesor Libre Facultad de Agronomía, Udelar, Presidente Academia Chilena Ciencias Agronómicas) ; Dr. Gerardo Gallego (Coordinador del Laboratorio de Biotecnología Vegetal del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dra. Elizabeth Hodson (Profesora emérita de la Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia); Dr. Paul Chavarriaga (líder de la plataforma de transformación genética y edición de genomas del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dr. Marco Dalla-Rizza (Coordinador de la Unidad de Biotecnología, Investigador principal referente, presidente REDBIO 2016-2020). |
Contenido : |
Potato is considered a staple food in the world and its production is limited by the presence of bacterial wilt (BW) disease caused by Ralstonia solanacearum. Host resistance is the most sustainable and cost-effective strategy to manage BW, although resistant commercial potato cultivars are not yet available. Our group incorporated the EFR receptor of Arabidopsis thaliana(AtEFR), which recognizes the elongation factor Tu, preserved in bacteria, triggering an immune response. AtEFR was tested in two genetic backgrounds: a susceptible commercial cultivar (INIA Iporá) and a breeding clone with partial resistance introgressed from Solanum commersonii. In this work, the effect of the AtEFR receptor on BW resistance was evaluated for the first time, using conditions resembling natural field infection. In addition, the colonization patterns of wild-type and transgenic lines were compared by using luminescent and fluorescent R. solanacearum reporter strains. Both approaches showed a delay and a decrease in the severity of wilting symptoms in the AtEFR-transformed genotypes. Differential colonization pat-terns were observed, revealing a higher bacterial development in the non-transformed plants. ThisAtEFR effect seems more pronounced in the interspecific breeding line, possibly leading to a more effective activation of the plant immune system. |
Palabras claves : |
BACTERIAL WILT; EF-Tu RECEPTOR; MARCHITEZ BACTERIANA; PAMP TRIGGERED IMMUNITY; PAPA TRANSGENICA; POLYGENIC RESISTANCE; RESISTENCIA MEDIADA POR PAMP; RESISTENCIA POLIGÉNICA; TRANSGENIC POTATO. |
Asunto categoría : |
H20 Enfermedades de las plantas |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14913/1/Fort-2020-Agrociencia.pdf
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Marc : |
LEADER 03834naa a2200361 a 4500 001 1061634 005 2021-03-12 008 2020 bl uuuu u00u1 u #d 024 7 $a10.31285/AGRO.24.413$2DOI 100 1 $aFORT, S. 245 $aPotato plants transformed with the Arabidopsis EF-Tu receptor (EFR) show restricted pathogen colonization and enhanced bacterial with resistance under conditions resembling natural field infections. [Plantas de papa transformadas con el receptor de EF-Tu (EFR) de Arabidopsis presentan una colonización restringida del patógeno y mayor resistencia a la marchitez Bacteriana bajo condiciones semejantes a la infección natural de campo].$h[electronic resource] 260 $c2020 300 $a16 p. 490 $a2301-1548 500 $aArticle history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020. Comité científico editor: Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad Nacional de la Plata, La Plata, Buenos Aires, Argentina); Dra. Alicia Castillo (Investigadora Principal, Unidad de Biotecnología de INIA, Uruguay) ; Dr. Juan Izquierdo (Profesor Libre Facultad de Agronomía, Udelar, Presidente Academia Chilena Ciencias Agronómicas) ; Dr. Gerardo Gallego (Coordinador del Laboratorio de Biotecnología Vegetal del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dra. Elizabeth Hodson (Profesora emérita de la Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia); Dr. Paul Chavarriaga (líder de la plataforma de transformación genética y edición de genomas del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dr. Marco Dalla-Rizza (Coordinador de la Unidad de Biotecnología, Investigador principal referente, presidente REDBIO 2016-2020). 520 $aPotato is considered a staple food in the world and its production is limited by the presence of bacterial wilt (BW) disease caused by Ralstonia solanacearum. Host resistance is the most sustainable and cost-effective strategy to manage BW, although resistant commercial potato cultivars are not yet available. Our group incorporated the EFR receptor of Arabidopsis thaliana(AtEFR), which recognizes the elongation factor Tu, preserved in bacteria, triggering an immune response. AtEFR was tested in two genetic backgrounds: a susceptible commercial cultivar (INIA Iporá) and a breeding clone with partial resistance introgressed from Solanum commersonii. In this work, the effect of the AtEFR receptor on BW resistance was evaluated for the first time, using conditions resembling natural field infection. In addition, the colonization patterns of wild-type and transgenic lines were compared by using luminescent and fluorescent R. solanacearum reporter strains. Both approaches showed a delay and a decrease in the severity of wilting symptoms in the AtEFR-transformed genotypes. Differential colonization pat-terns were observed, revealing a higher bacterial development in the non-transformed plants. ThisAtEFR effect seems more pronounced in the interspecific breeding line, possibly leading to a more effective activation of the plant immune system. 653 $aBACTERIAL WILT 653 $aEF-Tu RECEPTOR 653 $aMARCHITEZ BACTERIANA 653 $aPAMP TRIGGERED IMMUNITY 653 $aPAPA TRANSGENICA 653 $aPOLYGENIC RESISTANCE 653 $aRESISTENCIA MEDIADA POR PAMP 653 $aRESISTENCIA POLIGÉNICA 653 $aTRANSGENIC POTATO 700 1 $aFERREIRA, V. 700 1 $aMURCHIO, S. 700 1 $aSCHVARTZMAN, C. 700 1 $aGALVÁN, G.A. 700 1 $aVILARÓ, F. 700 1 $aSIRI, M.I. 700 1 $aDALLA RIZZA, M. 773 $tAgrociencia Uruguay 2020$gv. 24, no. NE2, Article 413. DOI: 10.31285/AGRO.24.413
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